The results of aeropalynological studies in Ivano-Frankivsk during 2013–2015 are presented in this article. The studies were conducted using a specially designed gravimetric Durham pollen trap installed at a height of 24 m from the ground. We determined that pollen grains of the following taxa dominate in the city’s aeropalynological spectrum: <em>Corylus</em>, <em>Alnus</em>, <em>Betula</em>, <em>Populus</em>, <em>Fraxinus</em>, <em>Salix</em>, <em>Carpinus</em>, <em>Quercus</em>, <em>Pinus</em>, <em>Picea</em>, Poaceae, Urticaceae, <em>Artemisia</em>, and <em>Ambrosia</em>. Pollen grains from woody plants dominate the aeropalynological spectrum. The considerable presence of the tree pollen grains in the ambient air is due to the geographical location of Ivano-Frankivsk in the forest-steppe zone of Ukraine. Pollen season of allergenic plants lasts from the second week of February to early October. The most unfavorable period for patients with pollen allergy in Ivano-Frankivsk is April, when there is a simultaneous appearance of high concentrations airborne allergenic pollen of <em>Betula</em>, <em>Fraxinus</em>, and <em>Carpinus</em>. From the second week of May to mid-July, high (&gt;50 pollen grains/m³) concentrations of allergenic pollen of the Poaceae family members are detected in the air. In the second half of August and early September, high concentrations of allergenic <em>Ambrosia </em>pollen were recorded in the city air. Conducting of pollen monitoring in the city is extremely necessary for prevention, diagnosis, and treatment of pollen allergy.