This study aimed to evaluate the physiological performance of white clover seed lots of different tegument colours, besides to analyses the electrical conductivity test methodology with different seeds number, water volume and soaking periods. The experiment was developed at the Seed Didactic Laboratory in the Agronomy College “Eliseu Maciel” at the Federal University of Pelotas, in Pelotas-RS, Brazil. White clover seeds were manually separate, composing four lots of different coloured seeds: yellow, orange, brown and mixed. White clover seeds of yellow colour tend to present greater physiological potential through its germination and vigour. The electrical conductivity test was not efficient on identifying different vigour levels in white clover seed lots.