Aim: This study was planned to determine the stress levels experienced by nursing students during the nursing education and the associated factors. Material and methods: This descriptive study was conducted between February and April 2018. The population of the study consisted of the students of The University Faculty of Health Sciences Nursing Department. There were 1,200 students in the faculty of nursing. The sample consisted of 479 students with 0.05 error, at 0.95 confidence interval, 0.6 effect size and 0.95 of population representation power with power analysis. Participants used Introductory Questionnaire, Nursing Education Stress Scale. Results: According to the results of this study, it was determined that the nursing students experience high level of stress and their demographic characteristics were affected by their education stress and practical stress and academic stress subscale scores. Conclusion: It was also determined that stress scores of the students who preferred the nursing profession willingly or liked it while studying and found the profession prestigious were lower.