Crown gall on rose was observed in greenhouse during year 2003-2005. The disease incidence was up to 28.3% and the disease was the severer in hydrophonics culture than that in soil. The typical gall symptom occurred mainly on the root and crown resulting in poor foliage, stunting, and fewer blossoms. Sixty-three rose cultivars were inoculated with Agrobacterium. tumefaciens isolated from rose crown gall, to evaluate rose cultivar-specific resistance. The size of galls from inoculated rose stems was measured in a greenhouse test. Tumors formed in almost varieties of rose inoculated. Based on the frequency of tumor occurrence and weight of galls formed on the stem of rose, it was shown that `Little Marble`, `Golden Gate` and `Rosa Rox-ette` were extremely susceptible to crown gall. Some varieties such as `Little Silver` appeared to be resistant to the crown gall.