Large discrepancies remain regarding the timing of Cenozoic paleoclimatic and paleoenvironmental transitions in the central Asia. The first order driving force behind these changes has been intensively debated. Global climate change, the uplift of Tibetan Plateau, and the evolution of Paratethys sea have been proposed as three major candidates. To understand the evolutionary history of climate and environment of the region and controlling factors responsible for these paleoclimatic and paleoenvironmental changes, a combined study utilizing multiple proxies, including microfossils, bulk carbonate carbon and oxygen isotopes, long chain alkenones, plant n-alkane-based indices (carbon preference index, average chain length and Paq) and compound-specific carbon and hydrogen isotopes of higher plant n-alkanes, was carried out on a long, continuous and well-dated section in Dahonggou, Qaidam Basin, northern Tibetan Plateau. A parallel study was also carried out in another relatively shorter section in the Xunhua Basin, northern Tibetan Plateau. Six intervals of paleoclimatic and paleoenvironmental transitions over an interval of ~35 Myr can be recognized in the studied sections, including Late Eocene-Early Oligocene gradual drying (prior to ~30 Ma), Middle Oligocene aridification (~30-26 Ma), Late Oligocene-Early Miocene wetting (~26-21 Ma), Early Miocene drying (~21-17 Ma), Middle Miocene climatic optimum (~17-13 Ma), and deteriorated climate since the late Middle Miocene (~13 Ma onwards). The reconstructed onsite C4 plant abundance including occurrence of C4 plants and their thriving and the followed decreasing, a sensitive indicator of available moisture level in the environment, agrees well with these intervals. Microfossils and long-chain alkenones suggest that a relic sea existed in the Qaidam Basin during the Middle Miocene, thus falsifying any hypothesis of significant variations in elevations of northern Tibetan Plateau prior to the Middle Miocene. The relatively stable elevations since the Eocene and before ...