Sesame is an oilseed crop that is growing in many developing countries for its good market value and new economic opportunities. However, its cultivation is however compromised by parasitic constraints which strongly reduce yields. This study was carried out on fungal strains associated with seeds and the effectiveness of a fungicide Mancomax. The detection of fungal strains associated with sesame was made using the blotting paper method. The efficiency of Mancomax 80 WP (Mancozeb) was evaluated on the seeds of four sesame accessions in order to evaluate its effectiveness in inhibiting the growth and development of seed-borne fungi. The contamination rate of the sesame varieties studied varies according to the accessions with a diversity of associated fungal strains. These fungi; especially Curvularia sp, Aspergillus sp, and Fusarium sp are fungi that produce mycotoxin that inhibits seed germination. The results show that this fungicide inhibits fungal infection to 100% for all concentrations studied for Mancomax effects on seed disinfection. Mancomax may be an ideal disinfectant to reduce the low germination rate of sesame seeds