<b>Objective</b>: <i>Nyctereutes procyonoides</i> immunoglobulin G (IgG) gene is partially cloned.<br /> <b>Material and methods</b>: In order to obtain a certain length (966bp) of <i>Nyctereutes procyonoides</i> immunoglobulin G (IgG), two pairs of primers are designed according to the conserved nucleotide sequence of canine (GenBank:AF354265, AF354265, AF354266, AF354267) and mink (GenBank: L07789). Using Bioinformatics technology and Western-blot to analyze antigenicity of <i>Nyctereutes procyonoides</i> IgG-B gene.<br /> <b>Results: </b> The homology for nucleotide sequence of IgG between <i>Nyctereutes procyonoides</i> and canine (IgG A, IgG B, IgG C, IgG D), mink, Homo sapiens, Oryctolagus cuniculus, Mus musculus, Anas platyrhynchos and gallus were respectively (88.1%, 93.6%, 85.4%, 87.2%), 83.7%, 74.8%, 71.8%, 69.2%, 51.6%, 48.4%. It can be seen that there was high homology of aminoacid sequence between IgG of <i>Nyctereutes procyonoides</i> and IgG (A, B, C, D) of canine. And the serum antibody of <i>Nyctereutes procyonoides</i> had obviously cross-reaction with HRP conjugated rabbit anti-dog IgG,compared with those of canine, oryctolagus cuniculus, mus musculus, mink, gallus.<br /> <b>Conclusions</b>: We successfully got <i>Nyctereutes procyonoides</i> immuneglobulin G (IgG) gene (GenBank: KM010191). There is the closest ties of consanguinity of IgG exist between <i>Nyctereutes procyonoides</i> and canine among the mammal through the genetic evolution.The detection and treament of canine distemper can be used on <i>Nyctereutes procyonoides</i>.