Ultrasound images can be utilized as a non-invasive method in the reproductive program. Striped catfish in various gonadal development were examined with a portable Mindray Model Z60Vet using the Convex probe at a frequency of 5 MHz. The results showed that the male gonads of immature striped catfishes were not distinguished from other visceral organs by using an ultrasonic technique. Ultrasound images in ovaries in stages II and III had significant differences. In ovary ‘s group of stage III, the oocyte’s dimensions on the ultrasound images were twice bigger than the group of stage II was. Before the premilitary injection, eggs displayed small, smooth, black dots, and 12h after the definitive dose, egg diameters raised twice and had brighter dots on the ultrasound images.