The aim of this study was to establish an experimental protocol for the artificial reproduction of royal carp ‘Cyprinus carpio’ with the hormonal induction of spawning. For this purpose, we conducted this study at an aquaculture farm in Khemis Meliana (Ain Defla, Algeria). The study included six royal carp broodstock, 4 females and 2 males. Hormone injection was performed in the dorsal muscle under the fin. The induction of carp was done successively in females by GnRH at a dose of 3 mg/kg and in males by hCG at a dose of 500 IU per kg weight. Fertilisation was done artificially by the dry method. After incubation of eggs, a binocular magnifying glass was used to check the condition of eggs and the development of the embryos over time. The results obtained show that the artificial reproduction of this species was possible, with success of ovulation and fertilisation, and hatching and growth of the larvae. Stripping was done after 24 h of hormonal stimulation at a temperature of 21°C. Disaggregation of eggs was performed efficiently with whole milk. The total number of eggs was 552,000 eggs with a weight of 1104 g. The average latency time was 22 hours. The hatching rate after 3 days of incubation on artificial spawning grounds at 21±1°C was 441,600 larvae (80%). Larvae food follow-up started from the 3rd day post-hatching with a combination natural food (rotifers) and artificial food. At the end of this experiment, we can conclude that it is possible to improve reproduction through the proper use of hormonal stimulation techniques and by improving feeding and abiotic factors that are dominant in fish farming.

Cilj je ovoga bio rada utvrditi eksperimentalni protokol u svezi umjetnog osjemenjivanja šarana ‘Cyprinus carpio’ s hormonalnom indukcijom mriještenja. U tu smo svrhu proveli studiju na akvakulturnoj farmi u Khemis Meliana (Ain Defla, Alžir). U ovom smo radu koristili šest šarana iz matičnog jata: 4 ženke i 2 mužjaka. Hormonalne injekcije dane su u dorzalni mišić ispod peraja. Indukcija šarana u ženki obavljena je sukcesivno putem GnRH pri dozi od 3 mg/ kg i mužjaka dozom hCG po stopi od 500 IU po kg mase. Oplodnja je obavljena umjetno, suhom metodom. Nakon inkubacije jajašaca za provjeru stanja jajašaca i razvoja embrija tijekom vremena rabljeno je binokularno povećalo. Dobiveni rezultati pokazuju da je umjetno osjemenjivanje ove vrste moguće, kao i preživljavanje i rast ličinki s uspješnom ovulacijom, oplodnjom i izlijeganjem ličinki. Prikupljanje zametnih stanica obavljeno je nakon 24 sata od hormonalne stimulacije na temperaturi od 21 °C. Deagregacija jajašaca učinkovito je obavljena pomoću punomasnog mlijeka. Ukupni broj jajašaca bio je 552,000 jajašaca s masom od 1104 g. Prosječno vrijeme latencije bilo je 22 sata. Postotak izlijeganja nakon 3 dana inkubacije na umjetnoj podlozi za mriještenje na temperaturi od 21 °C +/- 1 bio je 441,600 ličinki (80 %). Naknadna hranidba ličinki započela je trećeg dana od izlijeganja s diversifikacijom između prirodne hrane na bazi rotifera i umjetne hrane. Na kraju pokusa, možemo zaključiti da je moguće ispravnom uporabom tehnika hormonalne stimulacije i poboljšanjem hranidbe i abiotičkih čimbenika koji su dominantni u uzgoju ribe poboljšati reprodukciju.