Objective: The objective of this study is to assess the antimicrobial activity of marine actinobacteria isolated from coastal regions of Andhra Pradesh, India. Materials and Methods: Using sterile techniques, soil samples were collected from Machilipatnam (MCP), Krishna district, Andhra Pradesh, India. Biochemical tests were performed to identify isolated actinobacteria. Antimicrobial activity of the isolated organisms was performed by the disk plate technique. Results: In this study, six mangrove soil samples were collected and performed culture-dependent methods to obtain marine actinobacteria. A total of five actinobacteria were isolated in which two exhibited antibacterial as well as antifungal activity. Among the five, MCP-2 isolate had shown promising antibacterial and antifungal activity. Conclusion: The findings of this investigation revealed that the coastal marine actinobacteria are an important source of novel antibiotics