The aim of this study was to evaluate the acceptance of concentrates containing increasing levels of citrus pulp (7, 14, 21 and 28%) and the most preferred level of inclusion, observing the first action and the palatability using the preference test. Ten healthy mares, averaging 500 kg live weigh (LW), were housed in individual pens, where the four experimental concentrates were offered simultaneously, in a linear and continuous feeder for horses, for a period of 15 min. During this period, the first action (smelling or eating), the first choice (first concentrate effectively ingested) and the intake ratio (intake of each concentrate in relation to the total concentrate intake) were observed. Effect of inclusion on first action was observed (P<0.01), once 80% of animals fed promptly the concentrate and only 20% smelled it before the choice. The concentrate with 7% of citrus pulp presented the highest intake ratio (0.4425; P<0.01). For the first choice variable, there was no difference (P>0.05), however the concentrate with 7% was the most preferred (36.67%). Citrus pulp can be used as concentrate feed for horses. Therefore, low levels of inclusion in the concentrate, close to 7%, are recommended when the concentrate is based on corn, wheat bran, soybean meal, with no flavour agents like molasses.