Since the breed of origin highly affects the beef price, reliable methods are needed to detect incorrect declarations. As most breeds are standardised for coat colour, the Melanocortin 1 Receptor gene (MC1R), involved in the regulation of eu/pheomelanins synthesis, has been suggested as marker for breed traceability of products of animal origin. The aim of this investigation is to characterise the main breeds reared in the Piedmont Region by MC1R locus and to apply the analysis of the locus to breed traceability of beef cuts purchased in different outlets of the Region. A total of 168 DNA samples of four cattle breeds (Piemontese, Blonde d’Aquitaine, Italian Friesian and Aosta Red Pied) were analysed for MC1R locus by PCR-RFLP. In addition, 28 DNA samples from beef with breed indication were tested. Piemontese and Blonde d’Aquitaine were monomorphic for the E+and eallele, respectively. In the Friesian breed the EdEd genotype was the most frequent, but Edewas also observed (2%). Aosta Red Pied was the most variable breed, with the presence of the three alleles and five genotypes out of six. The comparison of the genotypic distribution in the four breeds clearly indicates that it is possible to distinguish among Piemontese, Blonde d’Aquitaine and Friesian breeds, but the same is not true for Aosta Red Pied, which has genotypes in common with the other breeds. The results on beef samples revealed a high percentage of mislabelling (about 18%), which concerned Friesian breed and crossbreds. These results indicate that MC1R locus is an effective marker in breed traceability of beef, when the involved breeds are characterised by different genotypes. Moreover, compared to other genetic markers, it has the great advantage of not requiring DNA reference sam- ples. This survey, though limited, has revealed a high percentage of incompatibilities. Therefore, the analysis of MC1R locus is recommended in the framework of product certification, at least for random controls within a system aimed at preventing fraud.