Wolf spiders (Arachneae: Lycosidae) are one of the successful hunters which extend their range through various habitats such as wetlands, springs, and sandy coasts, though less information is available on their feeding and predatory behaviour. During the course of regular field visits for an ecological study of benthic fauna on the Mahi estuarine mouth at Gulf of Khambhat, on the western coast of India (22°12’59.13”N, 72°36’55.47”E), interesting observations about the prey-predation relationship between the wolf spider (Lycos sp.) and two crab species Uca lactea annulipes and Cardisoma carnifex were recorded.