Chromosome analysis is very important part for the initial analysis of genetics. Some genetics abnormalities can be detected at the chromosome level and are usually associated with the inherited diseases. Accurate and prompt information need to be obtained for the purposes of the prevention of the genetics abnormalities genetics risk. This study was aimed to find out and to analyze the different size and morphological chromosome of Bali cattle and its crosses with Simmental cattle (Simbal cattle). Ten Simbal cattle (5 male and 5 female) and 5 female Bali cattle were used in this study. Five milliliter blood was collected using venous puncture through the jugular vein of each cattle. Chromosome was derived from white blood cells (lymphocyte) of peripheral blood. The Bali cattle and Simbal cattle have diploid chromosome (2n) of 60, with 29 pair of autosomes and one pair of sex chromosome. All autosomes are acrocentric with centromere index of 25.13 to 29.52% for Simbal cattle and 21.61 to 24.84% for Bali cattle. Sex chromosomes ware sub-metacentric in Simbal cattle either male or female and metacentric in female Bali cattle. Average length of chromosomes of female Simbal, male Simbal and female Bali cattle were 0.29 +/- 0.04 micron, 0.30 +/- 0.05 micron and 0.24 +/- 0.02 micron, respectively. Chromosome size of female Bali cattle was smaller than Simbal cattle.