The Afrotropical lutefish family Citharinidae (Citharinoidei, Characiformes) comprises three genera with eight species in total. Although Citharinidae have been studied in terms of taxonomy and systematics, no cytogenetic information was available for any representative of the family. Furthermore, only one species out of 116 in Citharinoidei (Distichodus affinis Günther, 1873) has been studied cytogenetically. Here, we report the karyotypes of Citharinus citharus (Geoffroy St. Hilaire, 1809) from West Africa and Citharinus latus Müller et Troschel, 1844 from Northeast Africa. The former has the diploid chromosome number 2n = 40 and the fundamental number FN = 80, while the latter has 2n = 44 and FN = 88. Hence, these karyotypes consist exclusively of bi-armed chromosomes. Such karyotypes were previously found in D. affinis and in many lineages of Neotropical species of another suborder of Characiformes, Characoidei. In contrast, the karyotypes dominated by uni-armed elements are typical for a number of phylogenetically basal lineages of Afrotropical and Neotropical Characoidei. We discuss the importance of our data on Citharinidae for the understanding of the karyotype evolution within the order Characiformes.