The pricing policy seems to be very important part of the strategy of the entire enterprise. The company’s position on a competitive market depends on the ability to make optimal pricing decisions. The article discusses the determinants of transport services’ prices and methods of their calculation (cost method, demand method and method based on competition). The main objective of the paper is the identification of the pricing determinants in the context of the rail transport. According to the authors, the most important determinants of rail transport services prices are mainly connected to prime cost, but also to factors classified into following groups: transportation enterprise, customer, market and competition. The introduction to the research was the examination of the largest rail operators in Poland and their pricing models.