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Applications in Plant Sciences
Article . 2014 . Peer-reviewed
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Applications in Plant Sciences
Article
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Applications in Plant Sciences
Article . 2014
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Easy Leaf Area: Automated digital image analysis for rapid and accurate measurement of leaf area

Authors: Easlon, Hsien Ming; Bloom, Arnold J;

Easy Leaf Area: Automated digital image analysis for rapid and accurate measurement of leaf area

Abstract

• Premise of the study: Measurement of leaf areas from digital photographs has traditionally required significant user input unless backgrounds are carefully masked. Easy Leaf Area was developed to batch process hundreds of Arabidopsis rosette images in minutes, removing background artifacts and saving results to a spreadsheet‐ready CSV file.• Methods and Results: Easy Leaf Area uses the color ratios of each pixel to distinguish leaves and calibration areas from their background and compares leaf pixel counts to a red calibration area to eliminate the need for camera distance calculations or manual ruler scale measurement that other software methods typically require. Leaf areas estimated by this software from images taken with a camera phone were more accurate than ImageJ estimates from flatbed scanner images.• Conclusions: Easy Leaf Area provides an easy‐to‐use method for rapid measurement of leaf area and nondestructive estimation of canopy area from digital images.

Country
United States
Keywords

580, Crop and Pasture Production, leaf area, QH301-705.5, digital images, Arabidopsis, Botany, Bioengineering, 004, QK1-989, Biology (General), Python

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    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    417
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 0.1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
417
Top 0.1%
Top 1%
Top 10%
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gold