This study investigated the punching shear behavior of full-scale, two-way concrete slabs reinforced with glass fiber reinforced polymer (GFRP) bars, which are known as noncorrosive reinforcement. The relatively low modulus of elasticity of GFRP bars affects the large deflection of flexural members, however, applying these to two-way concrete slabs can compensate the weakness of the flexural stiffness due to an arching action with supporting girders. The test results demonstrated that the two-way concrete slabs with GFRP bars satisfied the allowable deflection and crack width under the service load specified by the design specification even in the state of the minimum reinforcement ratio. Previous predicting equations and design equations largely overestimated the measured punching shear strength when the slab was supported by reinforced concrete (RC) girders. The strength difference can be explained by the fact that the flexural behavior of the supporting RC beam girders reduces the punching shear strength because of the additional deflection of RC beam girders. Therefore, for more realistic estimations of the punching shear strength of two-way concrete slabs with GFRP bars, the boundary conditions of the concrete slabs should be carefully considered. This is because the stiffness degradation of supporting RC beam girders may influence the punching shear strength.