In this study, the effects of habitat management practices on both pests and beneficial arthropods were evaluated in vineyards of North-eastern Italy through different field experiments: (1) mowing of inter-row spontaneous grasses in conventional and organic vineyards, (2) different timing of mowing of a green manure mixture, and (3) comparing different green manure mixtures. The first experiment followed a split-plot design, while randomized block design was used in the second and third experiment. In each experiment arthropods were sampled using different methods: leaf sampling, beating and sweep net sampling. Non-mowed spontaneous grasses in inter-rows of vineyards favored the abundance of natural enemies (e.g., predatory mites, parasitic wasps and spiders), and sometimes grapevine leafhoppers. Many arthropod species were recorded in higher numbers in organic vineyards. Late mowing of green manure favored beneficial arthropods (e.g., spiders and parasitic wasps), while it did not influence herbivore density. Groundcover management practices, aimed at increasing plant biodiversity in vineyards, could be a useful tool to enhance beneficial arthropod abundance, although the adoption of this practice should be carefully evaluated when pests occur. Semi-natural areas can contribute to create a more pest-stable agro-ecosystem and should be integrated with appropriate ecological infrastructures surrounding vineyards.