This research describes the results of the anaerobic digestion of gelatine as a potential hydrogen source with heat-shocked inoculum. The concentrations of applied gelatine were of VSS (volatile suspended solids) ranging from 10 g VSS/L to 30 g VSS/L. The initial process pH was 5.5, and, depending on the concentration, reached pH values from 7.5 to 7.8 after 55 days. Although the inoculum was heat-shocked in 30 g VSS/L of collagen, the process that occurred was hydrogenotrophic anaerobic digestion. In gelatine concentrations below 30 g VSS/L, hydrogen production was dominant only during the first 5 days of the experiments. Then, there was a change from dark fermentation to hydrogenotrophic methane production. The optimal hydrogen and methane yields resulted from the concentrations of 10 g VSS/L (7.65 mL ± 0.01 mL H2/g VSS and 3.49 ± 0.01 L CH4/g VSS). Additionally, 10 g VSS/L had the lowest accumulated emission of hydrogen sulphide (10.3 ± 0.01 mL of H2S), while 30 g VSS/L (0.440 ± 0.01mL H2S/g VSS) produced the lowest yield. After a lag time, the hydrogen production and hydrogen sulphide grew with a specific ratio, depending on the concentration. The hydrogen sulphide emission and sulphur added analysis proved that hydrogen sulphide originating from biogas created by bacteria remains longer than that from a substrate.