In this study, a new set of direct numerical simulations is generated and used to examine the influence of mixture composition heterogeneities on the propagation of a premixed iso-octane/air spherical turbulent flame, with a representative chemical description. The dynamic effects of both turbulence and combustion heterogeneities are considered, and their competition is assessed. The results of the turbulent homogeneous case are compared with those of heterogeneous cases which are characterized by multiple stratification length scales and segregation rates in the regime of a wrinkled flame. The comparison reveals that stratification does not alter turbulent flame behaviors such as the preferential alignment of the convex flame front with the direction of the compression. However, we find that the overall flame front propagation is slower in the presence of heterogeneities because of the differential on speed propagation. Furthermore, analysis of different displacement speed components is performed by taking multi-species formalism into account. This analysis shows that the global flame propagation front slows down due to the heterogeneities caused by the reaction mechanism and the differential diffusion accompanied by flame surface density variations. Quantification of the effects of each of these mechanisms shows that their intensity increases with the increase in stratification’s length scale and segregation rate.