In Colombia’s Orinoco, wildfires have a profound impact on ecosystem dynamics, particularly affecting savannas and forest–savanna transitions. Human activities have disrupted the natural fire regime, leading to increased wildfire frequency due to changes in land use, deforestation, and climate change. Despite extensive research on fire monitoring and prediction, the quantification of fuel accumulation, a critical factor in fire incidence, remains inadequately explored. This study addresses this gap by quantifying dead organic material (detritus) accumulation and identifying influencing factors. Using Brown transects across forests with varying fire intensities, we assessed fuel loads and characterized variables related to detritus accumulation over time. Employing factor analysis, principal components analysis, and a generalized linear mixed model, we determined the effects of various factors. Our findings reveal significant variations in biomass accumulation patterns influenced by factors such as thickness, wet and dry mass, density, gravity, porosity, and moisture content. Additionally, a decrease in fuel load over time was attributed to increased precipitation from three La Niña events. These insights enable more accurate fire predictions and inform targeted forest management strategies for fire prevention and mitigation, thereby enhancing our understanding of fire ecology in the Orinoco basin and guiding effective conservation practices.