Microplastic (MP) pollution is an emerging concern in ruminant production, as animals are exposed to MPs through air, water, and feeds. Ruminants play a key role in MP transmission to humans via animal products and contribute to MP return to agricultural soil through excreta. Identifying effective strategies to mitigate MP pollution in the ruminant sector is crucial. A promising yet understudied approach involves the potential ability of rumen microbiota to degrade MPs. This study investigated the in vitro ruminal degradation of three widely distributed MPs—low-density polyethylene (LDPE), polyethylene terephthalate (PET), and polyamide (PA)—over 24, 48, and 72 h. PET MP exhibited the highest degradation rates (24 h: 0.50 ± 0.070%; 48 h: 0.73 ± 0.057%; and 72 h: 0.96 ± 0.082%), followed by LDPE MP (24 h: 0.03 ± 0.020%; 48 h: 0.25 ± 0.053%; and 72 h: 0.56 ± 0.066%) and PA MP (24 h: 0.10 ± 0.045%; 48 h: 0.02 ± 0.015%; and 72 h: 0.14 ± 0.067%). These findings suggest that the ruminal environment could serve as a promising tool for LDPE, PET, and PA MPs degradation. Further research is needed to elucidate the mechanisms involved, potentially enhancing ruminants’ natural capacity to degrade MPs.