This study investigated the influence of starvation on the structure of the gut bacterial community in the Chinese white pine beetle (Dendroctonus armandi). A total of 14 operational taxonomic units (OTUs0.03) clusters belonging to nine genera were identified. Denaturing gradient gel electrophoresis (DGGE) profiles of bacterial PCR-amplified 16S rRNA gene fragments from the guts of starved male and female adults revealed that the bacterial community diversity increased after starvation. The dominant genus Citrobacter decreased significantly, whereas the genus Serratia increased in both starved female and starved male adults. The most predominant bacterial genus in D. armandi adults was Citrobacter, except for starved male adults, in which Serratia was the most abundant genus (27%). Our findings reveal that starvation affects gut bacterial dynamics in D. armandi, as has been observed in other insect species.