Hyperspectral images in the spectral wavelength range of 500 nm to 650 nm are used to detect green mold pathogens, which are parasitic on the surface of lemons. The images reveal that the spectral range of 500 nm to 560 nm is appropriate for detecting the early stage of development of the pathogen in the lemon, because the spectral intensity is proportional to the infection degree. Within the range, it was found that the dominant spectral wavelengths of the fresh lemon and the green mold pathogen are 580 nm and 550 nm, respectively, with the 550 nm being the most sensitive in detecting the pathogen with spectral imaging. The spectral intensity ratio of the infected lemon to the fresh one in the spectral range of 500 nm to 560 nm increases with the increasing degree of the infection. Therefore, the ratio can be used to effectively estimate the degree of lemons infecting by the green mold pathogens. It also shows that the sudden decrease of the spectral intensity corresponding to the dominant spectral wavelength of the fresh lemon, together with the neighboring spectral wavelengths can be used to classify fresh and contaminated lemons. The spectral intensity ratio of discriminating the fresh lemon from the infected one is calculated as 1.15.