Using microorganisms to enhance crop productivity is an active and increasing field of research, which encompasses the productive, environmental, and economic aspects of agricultural production to obtain high-quality crops with a reduction in the need for fertilizers. Among the nutrients necessary for plant growth, phosphorous is problematic due to its low availability and its susceptibility to convert into non-labile forms. In this regard, phosphate-solubilizing bacteria (PSB) can be an interesting tool to improve phosphorous availability and to reduce the requirements of phosphate fertilizers. This work aimed to evaluate the potential use of phosphate-solubilizing bacteria in the supply of phosphorus and soybean development. This study was conducted in the 2019/2020 and 2020/2021 harvests. The experimental design was in randomized blocks, containing seven treatments and six replicates. The treatments consisted of five doses of phosphate fertilization, using triple superphosphate fertilizer, associated with the application of Bacillus subtilis and Bacillus megaterium bacteria, and two treatments, with and without the use of phosphorous fertilizer and without the use of an inoculant. Plant tissue nutrients and biometric and productive parameters of the crop were assessed. According to the observed results, applying PSB associated with phosphate fertilization and phosphate fertilization alone did not influence soybean’s nutritional, biometric, and productive parameters in the two harvests. Thus, the application of B. subtilis and B. megaterium, either associated or not associated with phosphate fertilization, does not contribute to the nutrition, development, and yield of soybean crops in soil with a naturally low P content, considering the climatic and soil conditions of the study.