High-Throughput Sequencing Reveals Single Nucleotide Variants in Longer-Kernel Bread Wheat
Copyright policy )High-Throughput Sequencing Reveals Single Nucleotide Variants in Longer-Kernel Bread Wheat
The transcriptomes of bread wheat Yunong 201 and its ethyl methanesulfonate derivative Yunong 3114 were obtained by next-sequencing technology. Single nucleotide variants (SNVs) in the wheat strains were explored and compared. A total of 5907 and 6287 non-synonymous SNVs were acquired for Yunong 201 and 3114, respectively. A total of 4021 genes with SNVs were obtained. The genes that underwent non-synonymous SNVs were significantly involved in ATP binding, protein phosphorylation, and cellular protein metabolic process. The heat map analysis also indicated that most of these mutant genes were significantly differentially expressed at different developmental stages. The SNVs in these genes possibly contribute to the longer kernel length of Yunong 3114. Our data provide useful information on wheat transcriptome for future studies on wheat functional genomics. This study could also help in illustrating the gene functions of the non-synonymous SNVs of Yunong 201 and 3114.
- Murdoch University Australia
- Yunnan Agricultural University China (People's Republic of)
- Shenyang Agricultural University China (People's Republic of)
- Henan Agricultural University China (People's Republic of)
- Hunan Agricultural University China (People's Republic of)
single nucleotide variations (SNVs), Plant culture, Plant Science, SB1-1110, wheat, Mutation, Next-generation sequencing, next-generation sequencing, mutation, ethyl methanesulfonate (EMS), Transcriptome, transcriptome, Ethyl methanesulfonate (EMS)
single nucleotide variations (SNVs), Plant culture, Plant Science, SB1-1110, wheat, Mutation, Next-generation sequencing, next-generation sequencing, mutation, ethyl methanesulfonate (EMS), Transcriptome, transcriptome, Ethyl methanesulfonate (EMS)
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