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Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?

التباين الجيني لفيروس الهربس KOI في المختبر - حدث طبيعي ؟
Authors: Sandro Klafack; Qing Wang; Weiwei Zeng; Yingying Wang; Yingying Li; Suiping Zheng; J. Kempter; +3 Authors

Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?

Abstract

L'herpèsvirus de la carpe koï (KHV) dans le monde entier entraîne une mortalité élevée dans l'aquaculture de Cyprinus carpio L. Jusqu'à présent, on ne sait pas comment les différentes variantes du KHV se sont développées et comment elles se sont propagées chez les poissons, mais aussi dans les masses d'eau environnementales. Par conséquent, une méthode phylogénétique basée sur le nombre variable de répétitions en tandem (VNTR) a été améliorée pour obtenir des informations plus approfondies sur la phylogénie du KHV et sa distribution mondiale possible. De plus, un VNTR-3 qPCR a été conçu qui permet un typage rapide des virus. Cette étude présente une méthode utile pour le traçage moléculaire de divers types, variants et lignées de KHV.

El herpesvirus koi mundial (KHV) causa altas mortalidades en la acuicultura de Cyprinus carpio L. Hasta ahora, se desconoce cómo se han desarrollado las diferentes variantes de KHV y cómo se propagan en los peces, pero también en los cuerpos de agua ambientales. Por lo tanto, se mejoró un método filogenético basado en el número variable de repeticiones en tándem (VNTR) para obtener conocimientos más profundos sobre la filogenia del KHV y su posible distribución mundial. Además, se diseñó una qPCR de VNTR-3 que permite una rápida tipificación del virus. Este estudio presenta un método útil para el rastreo molecular de diversos tipos, variantes y linajes de KHV.

Worldwide koi herpesvirus (KHV) causes high mortalities in Cyprinus carpio L. aquaculture. So far, it is unknown how the different variants of KHV have developed and how they spread in the fish, but also in the environmental water bodies. Therefore, a phylogenetic method based on variable number of tandem repeats (VNTR) was improved to gain deeper insights into the phylogeny of KHV and its possible worldwide distribution. Moreover, a VNTR-3 qPCR was designed which allows fast virus typing. This study presents a useful method for molecular tracing of diverse KHV types, variants, and lineages.

يتسبب فيروس الهربس koi في جميع أنحاء العالم (KHV) في وفيات عالية في تربية الأحياء المائية Cyprinus carpio L. حتى الآن، من غير المعروف كيف تطورت المتغيرات المختلفة من KHV وكيف انتشرت في الأسماك، ولكن أيضًا في المسطحات المائية البيئية. لذلك، تم تحسين طريقة النشوء والتطور على أساس عدد متغير من التكرارات الترادفية (VNTR) لاكتساب رؤى أعمق في النشوء والتطور من KHV وتوزيعها المحتمل في جميع أنحاء العالم. علاوة على ذلك، تم تصميم VNTR -3 qPCR الذي يسمح بالكتابة السريعة للفيروسات. تقدم هذه الدراسة طريقة مفيدة للتتبع الجزيئي لأنواع ومتغيرات وأنساب فيروس التهاب الكبد الوبائي.

Keywords

Genotype, Innate Immunity to Viral Infection, sequence variation, VNTR, Immunology, Microbiology, Gene, Cyprinus carpio, KHV, Biochemistry, Genetics and Molecular Biology, Virology, molecular tracing, Genetics, RNA Sequencing Data Analysis, Molecular Biology, Biology, Immunological Responses in Aquatic Organisms, Immunology and Microbiology, FOS: Clinical medicine, Life Sciences, Variable number tandem repeat, Typing, Cyprinus, QR1-502, Virus, CyHV3, Phylogenetics, Fish, Fishery, FOS: Biological sciences, Phylogenetic tree

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    popularity
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    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
15
Top 10%
Average
Top 10%
Green
gold