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Characterization of Non-coding DNA Satellites Associated with Sweepoviruses (Genus Begomovirus, Geminiviridae) – Definition of a Distinct Class of Begomovirus-Associated Satellites

توصيف أقمار الحمض النووي غير المشفرة المرتبطة بالفيروسات الكاسحة (جنس الفيروس البيجوموفيري، Geminiviridae) – تعريف فئة مميزة من الأقمار الصناعية المرتبطة بالفيروس البيجوموفيري
Authors: Gloria Lozano; Helena P. Trenado; Elvira Fiallo‐Olivé; Dorys T. Chirinos; Francis Geraud‐Pouey; R. W. Briddon; Jesús Navas‐Castillo;

Characterization of Non-coding DNA Satellites Associated with Sweepoviruses (Genus Begomovirus, Geminiviridae) – Definition of a Distinct Class of Begomovirus-Associated Satellites

Abstract

Les bégomovirus (famille des Geminiviridae) sont des virus à ADN monocaténaire infectant les plantes et transmis par les aleurodes, qui causent des pertes de récoltes dans les régions les plus chaudes du monde. Les sweepovirus sont un groupe phylogénétiquement distinct de bégomovirus qui infectent les plantes de la famille des Convolvulaceae, y compris la patate douce (Ipomoea batatas). Deux classes de molécules subvirales sont souvent associées aux bégomovirus, en particulier dans l'Ancien Monde ; les bétasatellites et les alphasatellites. Une analyse d'échantillons de patate douce et d'Ipomoea indica d'Espagne et d'échantillons de Merremia dissecta du Venezuela a identifié de petites molécules sous-virales non codantes en association avec plusieurs sweepovirus distincts. Les séquences de 18 clones ont été obtenues et se sont avérées être structurellement similaires au virus-satellite du bouclage des feuilles de tomate (ToLCV-sat, le premier satellite d'ADN identifié en association avec un bégomovirus), avec une région ayant une identité de séquence significative avec la région conservée des bétasatellites, une séquence riche en A, une structure tige-boucle prédite contenant le nonanucléotide TAATATTAC, et une seconde tige-boucle prédite. Ces satellites associés au sweepovirus rejoignent un nombre croissant de satellites non codants de type ToLCV-sat identifiés récemment. Bien que partageant certaines caractéristiques avec les bêtasatellites, des preuves sont fournies pour suggérer que les satellites de type ToLCV-sat sont distincts des bêtasatellites et devraient être considérés comme une classe distincte de satellites, pour lesquels le nom collectif deltasatellites est proposé.

Los begomovirus (familia Geminiviridae) son virus de ADN monocatenario que se transmiten por la mosca blanca e infectan las plantas y causan pérdidas de cultivos en las partes más cálidas del mundo. Los sweepovirus son un grupo filogenéticamente distinto de begomovirus que infectan plantas de la familia Convolvulaceae, incluida la batata (Ipomoea batatas). Dos clases de moléculas subvirales a menudo se asocian con los begomovirus, particularmente en el Viejo Mundo; los betasatélites y los alfasatélites. Un análisis de muestras de batata e Ipomoea indica de España y muestras de Merremia dissecta de Venezuela identificó pequeñas moléculas subvirales no codificantes en asociación con varios sweepovirus distintos. Se obtuvieron las secuencias de 18 clones y se encontró que eran estructuralmente similares al virus-satélite del rizo de la hoja del tomate (ToLCV-sat, el primer satélite de ADN identificado en asociación con un begomovirus), con una región con identidad de secuencia significativa con la región conservada de betasatélites, una secuencia rica en A, una estructura de tallo-bucle predicha que contiene el no anucleótido TAATATTAC y un segundo tallo-bucle predicho. Estos satélites asociados a barrido de virus se unen a un número cada vez mayor de satélites no codificantes similares a ToLCV-sat identificados recientemente. Aunque comparten algunas características con los betasatélites, se proporcionan pruebas que sugieren que los satélites similares a los satélites ToLCV son distintos de los betasatélites y deben considerarse una clase separada de satélites, para la cual se propone el nombre colectivo deltasatélites.

Begomoviruses (family Geminiviridae) are whitefly-transmitted, plant-infecting single-stranded DNA viruses that cause crop losses throughout the warmer parts of the World. Sweepoviruses are a phylogenetically distinct group of begomoviruses that infect plants of the family Convolvulaceae, including sweet potato (Ipomoea batatas). Two classes of subviral molecules are often associated with begomoviruses, particularly in the Old World; the betasatellites and the alphasatellites. An analysis of sweet potato and Ipomoea indica samples from Spain and Merremia dissecta samples from Venezuela identified small non-coding subviral molecules in association with several distinct sweepoviruses. The sequences of 18 clones were obtained and found to be structurally similar to tomato leaf curl virus-satellite (ToLCV-sat, the first DNA satellite identified in association with a begomovirus), with a region with significant sequence identity to the conserved region of betasatellites, an A-rich sequence, a predicted stem-loop structure containing the nonanucleotide TAATATTAC, and a second predicted stem-loop. These sweepovirus-associated satellites join an increasing number of ToLCV-sat-like non-coding satellites identified recently. Although sharing some features with betasatellites, evidence is provided to suggest that the ToLCV-sat-like satellites are distinct from betasatellites and should be considered a separate class of satellites, for which the collective name deltasatellites is proposed.

فيروسات البيجوموفيروس (عائلة Geminiviridae) هي فيروسات الحمض النووي التي تنتقل عن طريق الذبابة البيضاء والتي تصيب النبات والتي تسبب خسائر في المحاصيل في جميع أنحاء الأجزاء الأكثر دفئًا من العالم. فيروسات الاجتياح هي مجموعة متميزة وراثيًا من فيروسات البيجوم التي تصيب نباتات العائلة Convolvulaceae، بما في ذلك البطاطا الحلوة (Ipomoea batatas). غالبًا ما ترتبط فئتان من الجزيئات دون الفيروسية بالفيروسات البيجومية، لا سيما في العالم القديم ؛ السواتل البيتا والفاساتيلات. حدد تحليل لعينات البطاطا الحلوة وإيبومويا إنديكا من إسبانيا وعينات ميريميا ديسكتا من فنزويلا جزيئات صغيرة غير مشفرة تحت الفيروس بالاشتراك مع العديد من فيروسات الكسح المتميزة. تم الحصول على تسلسلات 18 مستنسخًا ووجد أنها متشابهة هيكليًا مع قمر صناعي لفيروس تجعيد أوراق الطماطم (ToLCV - sat، أول قمر صناعي للحمض النووي تم تحديده بالاقتران مع فيروس بيغوموفيروس)، مع منطقة ذات هوية تسلسلية كبيرة للمنطقة المحفوظة من سواتل البيتا، وتسلسل غني بـ A، وهيكل حلقة جذعية متنبأ به يحتوي على TAATATTAC غير النوكليوتيد، وحلقة جذعية متوقعة ثانية. تنضم هذه الأقمار الصناعية المرتبطة بفيروس الاجتياح إلى عدد متزايد من الأقمار الصناعية غير المشفرة الشبيهة بـ ToLCV التي تم تحديدها مؤخرًا. على الرغم من مشاركة بعض الميزات مع الأقمار الصناعية بيتا، يتم تقديم أدلة تشير إلى أن الأقمار الصناعية الشبيهة بـ ToLCV - sat تختلف عن الأقمار الصناعية بيتا ويجب اعتبارها فئة منفصلة من الأقمار الصناعية، والتي يُقترح لها الاسم الجماعي دلتا الأقمار الصناعية.

Country
Spain
Keywords

Mechanisms of Plant Immune Response, Plant virus, sweet potato, Plant Science, Microbiology, Agricultural and Biological Sciences, Engineering, Virology, Genetics, Biology, sweepoviruses, Genomics and Pathogenicity of Plant Pathogenic Bacteria, Botany, Life Sciences, deltasatellites, QR1-502, Virus, Geminiviridae, Leaf curl, Aerospace engineering, Sweet potato, Satellite, Begomovirus, FOS: Biological sciences, Viral RNA Silencing and Plant Immunity, Geminiviruses, Ipomoea, DNA satellites, Merremia

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