Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
Copyright policy )Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of XylA and endogenous XKS1. Additional overexpression of genes RKI1, RPE1, TKL1, and TAL1 in the non-oxidative pentose phosphate pathway (PPP) in SyBE001 increased the xylose consumption rate by 1.19-fold. By repetitive adaptation, the xylose utilization rate was further increased by ∼10-fold in the resultant strain SyBE003. Gene expression analysis identified a variety of genes with significantly changed expression in the PPP, glycolysis and the tricarboxylic acid cycle in SyBE003.
- Hebei University China (People's Republic of)
- Collaborative Innovation Center of Chemical Science and Engineering Tianjin China (People's Republic of)
- TIANJIN UNIVERSITY China (People's Republic of)
- Tianjin University China (People's Republic of)
- Tianjin University China (People's Republic of)
xylose utilization, XylA, Xylose utilization, Xylose isomerase, yeast, Microbiology, xylose isomerase, QR1-502, evolutionary engineering, Synthetic Biology, synthetic biology
xylose utilization, XylA, Xylose utilization, Xylose isomerase, yeast, Microbiology, xylose isomerase, QR1-502, evolutionary engineering, Synthetic Biology, synthetic biology
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