The Stringent Response-Regulated sRNA Transcriptome of Borrelia burgdorferi
Copyright policy )The Stringent Response-Regulated sRNA Transcriptome of Borrelia burgdorferi
The Lyme disease spirochete Borrelia (Borreliella) burgdorferi must tolerate nutrient stress to persist in the tick phase of its enzootic life cycle. We previously found that the stringent response mediated by RelBbu globally regulates gene expression to facilitate persistence in the tick vector. Here, we show that RelBbu regulates the expression of a swath of small RNAs (sRNA), affecting 36% of previously identified sRNAs in B. burgdorferi. This is the first sRNA regulatory mechanism identified in any spirochete. Threefold more sRNAs were RelBbu-upregulated than downregulated during nutrient stress and included antisense, intergenic and 5' untranslated region sRNAs. RelBbu-regulated sRNAs associated with genes known to be important for host infection (bosR and dhhp) as well as persistence in the tick (glpF and hk1) were identified, suggesting potential mechanisms for post-transcriptional regulation of gene expression.
- University of Colorado Boulder United States
- Medical University of Vienna Austria
- University of Vienna Austria
- Center for Integrative Bioinformatics Vienna Austria
- MEDIZINISCHE UNIVERSITAT GRAZ Austria
EXPRESSION, BOSR, guanosine tetraphosphate and pentaphosphate, BINDING PROTEIN, MAMMALIAN INFECTION, Microbiology, Stringent response, DI-GMP, LYME-DISEASE SPIROCHETE, Cellular and Infection Microbiology, RPOS, SDG 3 - Good Health and Well-being, Stress, Physiological, 6S RNA, Lyme disease, small RNA, spirochetes, 106022 Mikrobiologie, GENE-REGULATION, Spirochetes, RNA sequencing, stringent response, Gene Expression Regulation, Bacterial, Small RNA, QR1-502, HISTIDINE KINASE HK1, Gene regulation, SDG 3 – Gesundheit und Wohlergehen, Borrelia burgdorferi, Guanosine tetraphosphate and pentaphosphate, 106022 Microbiology, RNA, Small Untranslated, gene regulation, Transcriptome
EXPRESSION, BOSR, guanosine tetraphosphate and pentaphosphate, BINDING PROTEIN, MAMMALIAN INFECTION, Microbiology, Stringent response, DI-GMP, LYME-DISEASE SPIROCHETE, Cellular and Infection Microbiology, RPOS, SDG 3 - Good Health and Well-being, Stress, Physiological, 6S RNA, Lyme disease, small RNA, spirochetes, 106022 Mikrobiologie, GENE-REGULATION, Spirochetes, RNA sequencing, stringent response, Gene Expression Regulation, Bacterial, Small RNA, QR1-502, HISTIDINE KINASE HK1, Gene regulation, SDG 3 – Gesundheit und Wohlergehen, Borrelia burgdorferi, Guanosine tetraphosphate and pentaphosphate, 106022 Microbiology, RNA, Small Untranslated, gene regulation, Transcriptome
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