Abstract Poultry litter from laying pens was added to a basal chicken breeder diet at levels of zero, two, and five percent and fed to S.C. White Leghorn hens in individual wire cages. Each diet was fed for 20 weeks to 81 hens divided equally among three experimental treatments. Weekly inseminations were made using 0.05 ml. of pooled semen. Fertility and hatchability data were kept on an individual hen basis. There were no significant differences among treatments for fertility or hatchability. Fertility varied from 89.8 percent for the zero poultry litter diet pens to 88.1 for both the two and five percent litter diet pens. Hatchability of fertile eggs was 80.4, 80.0, and 80.2 percent respectively, for hens fed diets containing zero, two, and five percent litter.