Bovine enterovirus (BEV) infection is a common viral disease in cattle. Although the infection is often subclinical, it is among the possible causes of gastroenteritis. BEV infections have also been associated with respiratory and reproductive system disorders and signs of diarrhea. BEV is divided into two serotypes, enterovirus serotype E (EV-E) and enterovirus serotype F (EV-F). This study aims to determine the seroprevalence of EV-E in calves in Konya province. For this purpose, 504 BEV unvaccinated calf blood serum samples from the Selcuk University Veterinary Faculty Virology Department laboratory were used. A serum neutralization test (SNT) was used to determine EV-E seroprevalence. 342 (67.85%) samples were found to be seropositive, and 162 (32.14%) were seronegative. In addition, as the serum neutralization 50 (SN50) antibody titers of seropositive animals were examined, the titers determined as 1/10, 1/15 and 1/20. Among the results we obtained, the highest antibody titers were defined as 1/80 and 1/120; the total number of animals with these values was In conclusion, bovine enteroviruses are an infection of importance for cattle breeding. Therefore, necessary precautions must be taken to protection infection. It is thought that the results of the present study will provide important data for future studies.