BACKGROUND: Psychosocial risks and work-related stress are one of the most complex, most significant and most challenging problems that organizations around the world are facing. Stress negatively affects all participants in the organization and significantly contributes to negative health consequences and economic outcomes. OBJECTIVE: With timely identification and adequate assessment of psychosocial risks, it is possible to create healthy workplaces and healthy organizations where employees are satisfied and motivated. The paper will present the results of the research of psychosocial risks on a large sample of respondents in the Republic of Serbia. METHODS: A questionnaire was distributed to 1,212 participants of which 1,140 answered all the questions asked. Data from the questionnaire were analyzed using multiple regression analysis, Kolmogorov-Smirnov test, Spearman’s correlation coefficient and Pearson’s chi-square test (χ2). RESULTS: The results of multiple regression analysis showed that all psychosocial risks taken together are statistically significantly (p < .001) and explain 24%of the variance of the criteria. CONCLUSIONS: Psychosocial risks identified as statistically significant predictors are Labor Demands, Interpersonal Relations, Lack of Support, Work Role and Job Uncertainty. All predictors are statistically significant predictors at the level.01.