Species categorizations can involve both scientific input and conservation questions about what should be preserved and how. We present a case study exploring the social construction of species categories using a real-life example of a cougar subspecies (Puma concolor stanleyana) purposefully introduced into Florida to prevent the functional extinction of a related subspecies of panther (P. c. coryi). Participants in an online sample (n = 500) were asked to make categorization decisions and then reflect on those decisions in an open format. Analysis of coded responses suggest people may experience “species” as both a social and biological construct, and that the question of what species people think an animal belongs to cannot be answered in isolation from questions about how that animal fits into larger social and biological systems.