Abstract Field experiments were conducted at nine locations in Texas and Georgia in 2005 and 2006 to evaluate peanut tolerance to lactofen. Lactofen at 220 g ai/ha plus crop oil concentrate was applied to peanut at 6 leaf (lf), 6 lf followed by (fb) 15 days after the initial treatment (DAIT), 15 DAIT alone, 6 lf fb 30 DAIT, 30 DAIT alone, 6 lf fb 45 DAIT, 45 DAIT alone, 6 lf fb 60 DAIT, and 60 DAIT alone in weed-free plots. Lactofen caused visible leaf bronzing at all locations. Yield loss was observed when applications were made 45 DAIT, a timing that would correspond to plants in the R5 (beginning seed) to R6 (full seed) stage of growth. At all locations except the Texas High Plains, this application timing was within the 90 d preharvest interval. Growers who apply lactofen early in the peanut growing season to small weeds should have confidence that yields will not be negatively impacted despite dramatic above-ground injury symptoms; however, applications made later in the season, during seed fill, may adversely affect yield.