Mosquitoes are public health concerns as vectors of arthropod-borne viruses (arboviruses) and/or as nuisances to humans, so surveillance efforts are important to determine areas and times that may pose a risk. The Horticulture Research Station (HRS), Ames, Iowa, consistently yields mosquitoes that are positive for West Nile virus (WNV). Objectives were to continue to assess human risk by monitoring both mosquitoes and sentinel chickens, which serve as vertebrate hosts for arbovirus.