Antisocial personality is generally used to describe people who treat other people with callous disregard and disrespect. Although animal cruelty is diagnostic of conduct disorder, a precursor of antisocial personality in children, it is unclear whether the term antisocial also encompasses disregard and disrespect toward non-human animals among adults. In this study we examined associations between three anti-animal attitudes and behaviors – speciesism, hunting/fishing, and lack of sympathy for animal rights – and traits that underlie adult antisocial behavior. We found consistent links between antisocial personality traits and anti-animal attitudes and behaviors in two samples. These effects were generally specific to traits linked to antisociality (although we also found associations with low openness), and these traits were stronger as predictors of variation among meat eaters than as predictors of differences between vegetarians and meat eaters. These results suggest that the term antisocial applies to negative attitudes and behaviors towards both humans and non-human animals and imply the potential value of considering anti-animal attitudes and behaviors in the clinical assessment of antisocial personality.