Nowadays, diabetes is a metabolic disease characterized by hyperglycemia by a problem in insulin secretion, insulin action, or both. It is known that oxidative damage plays an important role in the development of vascular and neurogenic complications of diabetes and antioxidants are effective on these pathologies. The aim of this study was to investigate the possible antidiabetic and antioxidant effects of Ferula elaeochytris root extract, commonly known as “ÇAKŞIR” in South and Southeastern Anatolia. Ferula elaeochytris was collected from the Engizek plateau of Kahramanmaraş. The plant root was prepared on a clean and dry surface and ground for grinding. The inhibitory effect of Ferula eleaochytris on glucose was determined electrochemically with the biosensor device, and total phenolic content was determined by Folin-Ciocalteu method as an indicator of antioxidant activity. In this study, according to electrochemical analysis with biosensor device, a linear decrease in glucose levels was observed depends on the decrease of enzyme-substrate interaction as a result of the addition of 100 µl cumulatively 1 minute (min) intervals of Ferula elaeochytris root extract (N=120). In addition, the total phenolic content of this plant was detected (41.45±2.38 mg GAE/g, n:3) by Folin-Ciocalteu method. It can be concluded that Ferula elaeochytris may have a hypoglycemic effect owing to its inhibitory effect on glucose levels and Ferula elaeochytris may also have a possible antioxidant source by means of the plant has a high capacity of total phenolic content. With the advanced studies to be done, it will be able to provide a more conscious utilization in accordance with the purpose of this unconsciously used plant by many people.