The study was based on macrofungi samples collected from the region within the boundaries of Muradiye district of Van province in 2014 and 2015. As a result of field and laboratory studies 86 macrofungi species belonging to 50 genera, 25 families, seven orders and three classes within the divisions Ascomycota and Basidiomycota were determined. The determined taxa are listed together with their habitats, substrates, collection locality, geographic coordinates and personel voucher numbers. One of them, Melanophyllum aematospermum (Bull.: Fr.) Kreisel, is a new record for Turkey as the first member of the genus Melanophyllum.