
doi: 10.2741/1696
pmid: 15970493
Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca++ transients in small neural networks. In the present review we give an overview of the current achievements and discuss emerging applications of the technique.
Neurons, Brain Mapping, Microscopy, Photons, 616.8, Photobleaching, Microscopy, Fluorescence, Microscopy, Fluorescence/*trends, Neurosciences, Neurons/*physiology, Neurosciences/*methods
Neurons, Brain Mapping, Microscopy, Photons, 616.8, Photobleaching, Microscopy, Fluorescence, Microscopy, Fluorescence/*trends, Neurosciences, Neurons/*physiology, Neurosciences/*methods
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