The Australian Sub-bottom Profiling Guidelines were developed by the AusSeabed community to establish a standardised approach to the acquisition of sub-bottom profiler data in an Australian context. They complement a suite of ocean best practice guidelines developed by the AusSeabed community including the Australian Multibeam Guidelines (eCat Record 121571) and the Australian Satellite Derived Bathymetry Guidelines.The guidelines provide recommended procedures for data acquisition, quality checking and data submission to the AusSeabed marine data portal. They were initially designed for use by the Australian Hydrographic Office Hydroscheme Industry Partnership Program (HIPP) to enable the acquisition of standardised, efficient and effective sub-bottom profile data for general seabed characterisation and collection of baseline data. Additionally, the guidelines may be used by any agency or party collecting seabed geophysical data in Australia’s marine jurisdiction for a range of use cases. The guidelines include a broad examination of data acquisition, basic processing for quality checking, metadata description, and guidance for data submission to AusSeabed. They do not include prescriptive equipment-specific hardware and software specifications, detailed user-defined settings or instrument preparation activities such as bench/workshop tests, personnel requirements, or provide survey costing information.