Soil salinisation is a serious land degradation issue worldwide, limiting the productivity of crops. Understanding the tolerance mechanisms employed by halophytes is of great value for ultimately identifying biomarkers for salt-tolerant crop breeding. This PhD project capitalised on the use of innovative membrane isolation technology Free-Flow Electrophoresis, next-generation proteomic technology SWATH-MS, and mass spectrometry-based lipidomic analyses to investigate changes in proteins and lipids in leaf tissue of the halophyte Mesembryanthemum crystallinum grown under salt conditions. Results provided a framework to develop a model for salinity-induced membrane remodelling at the subcellular level and will inform future work to engineer salt-tolerant crops.