Effects of faba bean and faba bean hulls on expression of selected genes in the small intestine of piglets
Copyright policy )Effects of faba bean and faba bean hulls on expression of selected genes in the small intestine of piglets
In a small intestinal segment perfusion (SISP) study in pigs, effects were studied of intestinal perfusion of ground faba beans (Vicia faba), faba bean hulls, or saline on intestinal net fluid absorption in intestinal segments either challenged or not with an enterotoxigenic Escherichia coli (ETEC). After an 8-h perfusion, piglets were euthanized and small intestinal mucosa samples were collected for analysis of expression level of a selected set of genes (APOC3, TIMP1, AQP8, MMP1, MUC13, and PAP) using real-time quantitative PCR (qPCR). Perfusion with ground faba beans and faba bean hulls and challenge with ETEC affected (P < 0.05) expression of several of genes in the intestinal mucosa. Expression of APOC3, TIMP1, AQP8, MMP1, and PAP was correlated with net fluid absorption in the small intestine of pigs.
- Wageningen University & Research Netherlands
Gene expression small intestine, Swine, Fluid absorption, Real-Time Polymerase Chain Reaction, Animal Feed, Diet, Vicia faba, Gene Expression Regulation, Intestine, Small, Animals, Pigs, Animal Nutritional Physiological Phenomena, Faba beans, Intestinal Mucosa
Gene expression small intestine, Swine, Fluid absorption, Real-Time Polymerase Chain Reaction, Animal Feed, Diet, Vicia faba, Gene Expression Regulation, Intestine, Small, Animals, Pigs, Animal Nutritional Physiological Phenomena, Faba beans, Intestinal Mucosa
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