Objective: The aim of this study was to evaluate the behavior of cupuaçu tree (Theobroma grandiflorum) seedlings as a function of agro-industrial waste. Method: The influence of agro-industrial waste on the development of cupuaçuzeiro seedlings was evaluated. Treatments included different proportions of organic waste (açaí ash, dendê compost and cattle manure) in proportions of 5%, 10%, 15% and 20% in relation to 3 kg of soil. Finally, morphological and physiological variables were determined. Result and Conclusion: The experimental data was analyzed using analysis of variance. Significant means were then compared using the Tukey test, with a significance level of 5%. From the results obtained, it was observed that one of the agro-industrial residues has the potential to act as an alternative substrate in the production of Theobroma grandiflorum seedlings. Researchimplications: The Theobroma grandiflorum species stands out as being extremely important, since its products and by-products are widely accepted in domestic and foreign markets, as well as contributing to Agroforestry Systems. In addition, there is a need to study alternative substrates, especially those generated on a large scale, considering the need to promote proper disposal with sustainable use. Originality/value: In the current scenario, with regard to the destination and final disposal of solid waste, there is a need to look for sustainable alternatives aimed at reusing this waste, especially in agriculture.