The genus Rubus is one of the most diverse in morphological and genetic terms, presenting a wide spectrum of wild and cultivated species that are desired for their edible fruits. In Ecuador, the cultivation of blackberry (R. glaucus and Rubus sp.) is presented along the inter-Andean alley, between 2,000 and 3,100 meters above sea level. This fruit is an economically important crop because of its high demand for fresh and processed consumption. This research was carried out at the Tumbaco Experimental Farm of INIAP (Ecuador) with the objective of finding characters to differentiate morphologically, phenologically and pomologically four cultivars of blackberry (Castilla, Andimora, Colombiana and Brazos). Three clusters (C) were formed by multivariate analysis. The cultivars Andimora and Colombiana (C3) do not have thorns, unlike Castilla (C2) and Brazos (C1), which have thorns. Colombiana was the earliest cultivar (161 days from sprouting to harvest) and Castilla the latest (186 days). Brazos had the highest fruit weight (5.85 g). Andimora had the highest soluble solids content (11.86 °Brix) and firm fruit. The differentiating characters observed in this study allow establishing parameters of distinction among the cultivars currently grown in Ecuador.