In the present study, the EPR spectroscopy was used to evidence differences in fruits of organically and conventionally grown bananas belonging to musaceae family. If in the investigated samples would be detected specific changes related to paramagnetic resonant centers, these could be regarded as a spectroscopic fingerprint in the differentiation of the organic and conventional fruits and vegetables. The EPR spectra were recorded from freeze-dried shell and pulp samples. The main paramagnetic species (iron, manganese and native semiquinone free radical) delivered for the investigated samples slight different EPR signals. In this stage, the results obtained by EPR testing put in evidence sensible differences between the two classes of samples, and draw the attention on differences in EPR signals recorded from banana pulp and shell.