This study was carried out to determine ability of secondary buds to winter freezing in two Vitis species viz. Vitis vinifera L. and Vitis labrusca L. In 2016 and 2017, one genotype of V. vinifera and one genotype of V. labrusca were sampled at 3 different periods during winter dormant period. The one-year-old shoots obtained from vineyards in winter and the secondary buds on 4th, 5th and 6th nodes on shoots has been used for freezing test. For freezing test, DTA (Differantial Thermal Analyses) methods has been used and test was started at +4 oC and temperature has been decreased and terminated at -30 oC. According to test results, the highest and the lowest temperatures to start freezing of secondary buds of the genotype belongs to V. vinifera was -16.48 oC and -19.49 oC, respectively. For V. labrusca genotype, these values were -15.77 oC and -20.99 oC, respectively. The short period of exotherm has been occurred in deep dormancy period for both species. As a result, freezing points of secondary buds varied among species as well years and physiological status of plants. Determining of freezing points of secondary buds in grapes, it is possible to make better prediction of winter injury.