Two new genera of Dactylogyrinae, Trinidactylus cichlasomatis gen. et sp. n. from the gills of Cichlasoma bimaculatum (L.) and Trinigyrus hypostomatis gen. et sp. n. from the gills of Hypostoma robinii (Valenciennes), are described. Curvianchoratus hexacleidus gen. et sp. n. is described from the gills of Curimata argentea (Gill) and placed in Curvianchoratinae subf. n. Price (1966), in an effort to simplify the taxonomy of the Dactylogyridae, proposed four subfamilies (Anacanthorinae, Dactylogyrinae, Heteronchocleidinae, and Ancyrocephalinae). Most genera listed from freshwater fishes in the Neotropical region (Mizelle and Kritsky, 1967, 1969; Mizelle, Kritsky, and Crane, 1968; Mizelle and Price, 1964; Price, 1967) belong to the Ancyrocephalinae. In the present study two new genera of Dactylogyrinae, Trinidactylus gen. n. and Trinigyrus gen. n., are described. Curvianchoratus gen. n. is proposed and placed in Curvianchoratinae subf. n. Materials, methods, and acknowledgments are identical to those given in Molnar, Hanek, and Fernando (1974).