The status of Aesculus pavia var. flavescens, a set of yellow-flowered populations endemic to the Edwards Plateau of central Texas, was evaluated on the basis of comparative morphology, geography, flowering phenology, and pollination ecology. Plants of typical A. pavia have red, tubular flowers with exserted stamens and are effectively pollinated by ruby-throated hummingbirds. Plants of var. flavescens have yellow, campanulate flowers with included stamens that can be pollinated effectively by large bees. These ethological reproductive isolating mechanisms are further augmented by a difference in flowering time between the two sets of populations and by geographical restriction of the yellow-flowered plants to localities on the Edwards Plateau. The yellow and red-flowered plants constitute two morphologically distinct groups, despite evidence of limited introgression in counties along the Balcones Escarpment. A yellow-flowered plant discovered in coastal east Texas may indicate the mechanism by which var. flavescens originated, but this plant is much more closely comparable to typical red-flowered var. pavia than to the yellow-flowered plants on the Edwards Plateau. An evolutionary history of section Pavia of Aesculus is presented, and it is concluded that the yellow-flowered plants of central Texas are best regarded as a taxonomic variety.